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yxeQ [2020-07-13 09:15:16]
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yxeQ [2020-07-13 09:15:16]

cis-sconitate decarboxylase
locus
BSU_39460
pI
5.94
mw
47.64 kDa
protein length
445 aa Sequence Blast
gene length
1338 bp Sequence Blast
function
metabolite conversion
product
cis-sconitate decarboxylase
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

    • Gene

    Coordinates
    4,055,536 4,056,873

    The protein

    Catalyzed reaction/ biological activity

  • cis-aconitate --> itaconate + CO2 [pubmed|32647315]

    • Protein family

  • prpD family (with [protein|602EAB591A550F68C14F35F6E5F0A91C3B997343|MmgE], according to UniProt)

    • Structure

  • [PDB|7BRA] [pubmed|32647315]

    • Expression and Regulation

    Operons

    genes
    [gene|19E6EF9CCA31440EAC046F8D6CA98B6A08589D58|yxeK]-[gene|EBC2B9107AD50DD8F8174FC0BE4AD90DEE6A7CCA|snaB]-[gene|D96EEDABB73AC66E6AB772431819E7C767DC8B9A|yxeM]-[gene|FFD5F07480A0E5A74852C69C436EE289BA72FC7F|yxeN]-[gene|F8C800CF71D21A1F3265977C4F0EE191D0FFE36A|yxeO]-[gene|C99AA3FDBB561BCED60915078DC746690C071753|sndB]-[gene|7DDB283173267AA106DC277787AAAB4F4F9975F4|yxeQ]
    description
    [Pubmed|10746760]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|16513748], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|50930C56C27D22715620A350220E3C56ADB41020|CymR]: repression, [Pubmed|16513748], in [regulon|50930C56C27D22715620A350220E3C56ADB41020|CymR regulon]

    • regulation

  • repressed in the presence of cysteine ([protein|50930C56C27D22715620A350220E3C56ADB41020|CymR]) [Pubmed|16513748]

    • additional information

  • the amount of the mRNA is substantially decreased upon depletion of [gene|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [PubMed|21815947]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-B713 (yxeQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1712 NBRP B. subtilis, Japan]
  • BKE39460 ([gene|7DDB283173267AA106DC277787AAAB4F4F9975F4|yxeQ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE39460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ACCTGCTGTCAGCCCTTGTT, downstream forward: _UP4_TAAGATCAACTCTATTCGGT
  • BKK39460 ([gene|7DDB283173267AA106DC277787AAAB4F4F9975F4|yxeQ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK39460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ACCTGCTGTCAGCCCTTGTT, downstream forward: _UP4_TAAGATCAACTCTATTCGGT

    • References

  • 16513748,29626092,16934291,32647315